3kb amplification

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  • PCR Master Mix

    with [ 7.5 | 12.5 ] mM MgCl2

    For in vitro use only

    PACK SIZES : 1ml

    Download Protocol

    Description:

    5x FIREPol® Master Mix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water).

    We recommend using 5x FIREPol® Master Mix in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining.

  • PCR Master Mix Ready to Load

    with [ 7.5 | 12.5 ] mM MgCl2

    For in vitro use only

    PACK SIZES : 1ml

    Download Protocol

     

    Description:

    5x FIREPol® Master Mix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water). Ready to Load format also includes a compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.

    We recommend using 5x FIREPol® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. 5x FIREPol® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectro-photometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.

     

  • Taq DNA Polymerase

    For in vitro use only

    PACK SIZES : 500U | 1000U | 2000U

     

    Description:

    FIREPol® is a highly processive, thermostable DNA polymerase. Due to its genetic modifications FIREPol® has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.