High Resolution Melt (HRM)
– HOT FIREPol® DNA Polymerase
– 5x EvaGreen® HRM buffer
– 12.5 mM MgCl2 1x PCR solution – 2.5 mM
– EvaGreen® dye
– ROX dye
EvaGreen® is a DNA-binding dye with many features that make it a superior for HRM. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!
Shipping and Storage conditions:
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® HRM Mix.