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EvaGreen® HRM Mix

For in vitro use only

PACK SIZES : 1ml | 8ml | 20ml

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Description:

5x HOT FIREPol® EvaGreen® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers.

HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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Description

Applications:

High Resolution Melt (HRM)

 

Mix Composition:

– HOT FIREPol® DNA Polymerase

– 5x EvaGreen® HRM buffer

– 12.5 mM MgCl2 1x PCR solution – 2.5 mM

– dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP 

– EvaGreen® dye

– BSA

 

EvaGreen® Dye:

EvaGreen® is a DNA-binding dye with many features that make it a superior for HRM. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!

 

Shipping and Storage conditions:

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® HRM Mix.

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