FIREScript Reverse Transcriptase

Applications:

– First strand cDNA synthesis

– RT-PCR

– RT-qPCR

Concentration:

200 U/µl

Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25 ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Reagents Provided:

– FIREScript Reverse Transcriptase  

– 10x RT Reaction Buffer with DTT

  500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl₂, 100 mM DTT

Shipping and Storage Conditions:

Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.