FIREScript RT cDNA synthesis KIT

For in vitro use only

Pack Size: 50 / 200 Reactions

Description:

FIREScript RT cDNA Synthesis Kit contains all the reagents you need to synthesize cDNA in 7 separate vials. Its fast reaction speed allows you to perform your synthesis in just 15 minutes while the high working temperature of the enzyme (up to 60 ºC) ensures high yield and great sensitivity. The kit format allows flexibility including the preincubation of complex RNA.

Description

Applications:

– First strand cDNA synthesis

– RT-PCR

– RT-qPCR

Concentration:

200 U/µl

Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25 ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Reagents Provided:

– FIREScript Reverse Transcriptase

– RiboGrip RNase Inhibitor

– 10x RT Reaction Buffer with DTT

500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl₂, 100 mMDTT

– Oligo (dT) Primer

– Random Primers

– dNTP Mix

– Water, nuclease free

Shipping and Storage Conditions:

Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.