Description
Applications:
– First strand cDNA synthesis
– RT-PCR
– RT-qPCR
Concentration:
200 U/µl
Storage and Dilution Buffer:
50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25 ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Reagents Provided:
– FIREScript Reverse Transcriptase
– RiboGrip RNase Inhibitor
– 10x RT Reaction Buffer with DTT
500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT
– Oligo (dT) Primer
– Random Primers
– dNTP Mix
– Water, nuclease free
Shipping and Storage Conditions:
Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.