Description
Applications:
– First strand cDNA synthesis
– RT-PCR
– RT-qPCR
Storage and Dilution Buffer:
50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25 ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Reagents Provided in Product Sample:
– FIREScript Enzyme Mix
FIREScript RT and RiboGrip RNase Inhibitor
– 10x RT Reaction Premix with Oligo (dT) and Random primers
RT Reaction Buffer with DTT, dNTPs, Oligo (dT) primer and Random primers
– 10x RT Reaction Premix with Oligo (dT) primer
RT Reaction Buffer with DTT, dNTPs and Oligo (dT) primer
– 10x RT Reaction Premix with Random primers
RT Reaction Buffer with DTT, dNTPs and Random primers
– 10x RT Reaction Premix without primers
RT Reaction Buffer with DTT and dNTPs
– Water, nuclease free
Reagents Provided:
– FIREScript Enzyme Mix
FIREScript RT and RiboGrip RNase Inhibitor
– 10x RT Reaction Premix
RT Reaction Buffer with DTT, dNTPs and priming method of choice*
– Water, nuclease free
Shipping and Storage Conditions:
Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
