Hot Start Blend Master Mix Ready to Load

with [ 7.5 | 10 | 12.5 | 15 ] mM MgCl2

For in vitro use only

PACK SIZES : 1ml | 20ml

Download Protocol




5x HOT FIREPol® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water), additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.

HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to five fold) compared to regular Taq polymerase.

In addition, the mix also allows amplification of longer fragments.

Generated PCR products are compatible with blunt-end cloning procedures.



– Hot Start PCR


Mix Composition:

HOT FIREPol® DNA polymerase

– Proofreading enzyme

– 5x Blend Master Mix Buffer

– 7. 5 mM MgCl2  1x PCR solution – 1.5 mM MgCl2

– 2 mM dNTPs of each  1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP


– Blue dye Migration equivalent to 3.5-4.5 kb DNA fragment

– Yellow dye Migration rate in excess of primers in 1% agarose gel: <35-45 bp

– Compound that increases sample density for direct loading

Advantages of Ready-To-Load Mix:

– decreased set-up time

– reduced risk of contamination

– less pipetting mistakes

– consistent reaction to reaction performance

– contains all reagents required for PCR

Like all our products, the 5x HOT FIREPol® Blend Master Mix is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.

Shipping and Storage Conditions:

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 12 months at 2-8ºC has no detrimental effects on the quality of 5x HOT FIREPol® Blend Master Mix Ready to Load.


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