Hot-Start DNA Polymerase

Applications:

– Hot Start PCR

– Primer extension

– TA cloning

Concentration:

5 U/µl

Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Reagents Provided:

– HOT FIREPol^® DNA Polymerase  

– HOT FIREPol^® 10x Buffer B1 (Mg²⁺, detergent free)  Tris-HCl and (NH₄)₂SO₄

– HOT FIREPol^® 10x Buffer B2 (Mg²⁺ free) Tris-HCl, (NH₄)₂SO₄ and detergent

– 25 mM MgCl₂

– 10x Solution S Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution).
Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.

Shipping and Storage Conditions:

Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol^® DNA Polymerase.