Hot Start PCR GC Master Mix

For in vitro use only

PACK SIZES : 1 ml | 20 ml

Download Protocol



5x HOT FIREPol® GC Master Mix is designed to provide highly specific high-yield amplification of GC-rich templates. Master Mix is a ready-to-use solution containing all reagents required for PCR (except template, primers and water). 100% DMSO and 25 mM MgCl2 are included in a package in separated vials.
Generated PCR products are compatible with TA cloning procedures



– Hot Start GC-rich PCR

– Fragment analysis

– TA cloning


Mix Composition:

– HOT FIREPol® DNA polymerase

– HOT FIREPol® GC Buffer

– 7.5 mM MgCl2 1x PCR solution – 1.5 mM MgCl2

– 2 mM dNTPs

– BSA 


In separate vial:

100% DMSO

– 25 mM MgCl2


Advantages of Master Mix:

– decreased set-up time

– reduced risk of contamination

– less pipetting mistakes

– consistent reaction to reaction performance

– contains all reagents required for PCR

Like all our products, the 5x HOT FIREPol® GC Master Mix is stable at ambient temperature (up to 37°C) for one month. This makes handling and set-up even more convenient, since no ice has to be used.

Product Performance:

The unique composition of the mix supports robust amplification of broad range of GC-rich targets. A total 14 GC-rich genes were amplified from human genomic DNA, using recommended reaction set-up and cycling protocol for 5x HOT FIREPol® GC Master Mix.











Mix performs well with GC-rich  amplicons up to 3000 bp.

Good results are obtained also with Fragment Analyzis (DAT/SLC6A3).


Shipping and Storage Conditions:

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of 5x HOT FIREPol® GC Master Mix.


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