PCR Master Mix Ready to Load

with [ 7.5 | 12.5 ] mM MgCl2

For in vitro use only


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5x FIREPol® Master Mix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water). Ready to Load format also includes a compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.

We recommend using 5x FIREPol® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. 5x FIREPol® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectro-photometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.


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– Suited for a wide range of PCR assays

– Primer extension


Reagent Composition:

– FIREPol® DNA polymerase

– 5x Reaction Buffer B 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20 

– 7.5 mM MgCl2 1x PCR solution – 1.5 mM MgCl2 

– 2 mM dNTPs of each  1x PCR solution – 200 µM dATP, 200 µM dCTP,   200 µM dGTP and 200 µM dTTP

Blue dye Migration equivalent to 3.5-4.5 kb DNA fragment

– Yellow dye  Migration rate in excess of primers in 1% agarose gel: <35-45 bp

– Compound that increases sample density for direct loading

Advantages of Ready-to-Load Mix

– decreased set-up time

– reduced risk of contamination

– less pipetting mistakes

– consistent reaction to reaction performance

– contains all reagents required for PCR

Like all our products, the 5x FIREPol® Master Mix Ready to Load is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.

Shipping and Storage Conditions:

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8ºC has no detrimental effects on the quality of FIREPol® 5x Master Mix Ready to Load.


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