Probe qPCR Mix Plus (no ROX)

For in vitro use only

PACK SIZES : 1ml | 8ml | 20ml

Download Protocol

 

Description:

HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative  PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.
HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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Description

Applications:

– Detection and quantification of DNA and cDNA targets

– Profiling gene expression

– Microbial detection

– Viral load determination

 

Mix Compatibility:

Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™, Chromo4™, Opticon® 2 & MiniOpticon®

Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000

Eppendorf: Mastercycler®: ep realplex2 & ep realplex4  

Illumina: The Eco™   

Roche: LightCycler® 480

Mix Composition:

– HOT FIREPol® DNA Polymerase

– 5x Probe qPCR buffer

– 15 mM MgCl2 1x PCR solution – 3 mM   

– dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP 

– No ROX dye

Shipping and Storage Conditions:

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® Probe qPCR Mix Plus (no ROX).

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