Description
Applications:
– Detection and quantification of DNA and cDNA targets
– Profiling gene expression
– Microbial detection
– Viral load determination
Mix Compatibility:
– Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™, Chromo4™, Opticon® 2 & MiniOpticon®
– Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000
– Eppendorf: Mastercycler®: ep realplex2 & ep realplex4
– Illumina: The Eco™
– Roche: LightCycler® 480
Mix Composition:
– HOT FIREPol® DNA Polymerase
– 5x Probe qPCR buffer
– 15 mM MgCl2 1x PCR solution – 3 mM
– dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP
– No ROX dye
Shipping and Storage Conditions:
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® Probe qPCR Mix Plus (no ROX).
Reviews
There are no reviews yet.