Hot Start PCR

Showing all 4 results

  • Hot Start Blend Master Mix


    with [ 7.5 | 10 | 12.5 | 15 ] mM MgCl2

    For in vitro use only

    PACK SIZES : 1ml | 20ml

    Download Protocol

     

    Description:

    5x HOT FIREPol® Blend Master Mix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water).

    HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to five fold) compared to regular Taq polymerase.

    In addition, the mix also allows amplification of longer fragments.

    Generated PCR products are compatible with blunt-end cloning procedures.

  • Hot Start Blend Master Mix Ready to Load


    with [ 7.5 | 10 | 12.5 | 15 ] mM MgCl2

    For in vitro use only

    PACK SIZES : 1ml | 20ml

    Download Protocol

     

     

    Description:

    5x HOT FIREPol® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water), additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.

    HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to five fold) compared to regular Taq polymerase.

    In addition, the mix also allows amplification of longer fragments.

    Generated PCR products are compatible with blunt-end cloning procedures.

  • Hot Start PCR GC Master Mix


    For in vitro use only

    PACK SIZES : 1 ml | 20 ml

    Download Protocol

     

    Description:

    5x HOT FIREPol® GC Master Mix is designed to provide highly specific high-yield amplification of GC-rich templates. Master Mix is a ready-to-use solution containing all reagents required for PCR (except template, primers and water). 100% DMSO and 25 mM MgCl2 are included in a package in separated vials.
    Generated PCR products are compatible with TA cloning procedures
  • Hot-Start DNA Polymerase


    HOT START DNA Polymerase

    For in vitro use only

    PACK SIZES : 500U | 1000U

     

    Description:

    HOT FIREPol® DNA Polymerase is a chemically modified FIREPol® DNA Polymerase. HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extentension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR setup. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.