qPCR

Reagents needed for real-time quantitative PCR

Showing all 6 results

  • EvaGreen® qPCR Mix Plus (no ROX)


    For in vitro use only

    PACK SIZES : 1ml | 8ml | 20ml

    Download Protocol

     

    Description:

    HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers.

    HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

  • EvaGreen® qPCR Mix Plus (ROX)


    For in vitro use only

    PACK SIZES : 1ml | 8ml | 20ml

    Download Protocol

    Description:

    HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye. The user simply needs to add water, template and primers.
    HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

  • EvaGreen® qPCR Supermix


    Suitable for ROX-dependent and ROX-independent qPCR cyclers

    For in vitro use only

    PACK SIZES : 1ml | 8ml | 20ml

    Download Protocol

    Description:

    HOT FIREPol® EvaGreen® qPCR Supermix is an optimised ready-to-use solution for real-time quantitative PCR assays, including EvaGreen® dye. It comprises all the components necessary to perform qPCR excluding the template and primers, to perform highly sensitive qPCR.
    HOT FIREPol®DNA Polymerase is activated by a 12 min incubation step at 95°C. Hot-start mechanism prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

  • Probe qPCR Mix Plus (no ROX)


    For in vitro use only

    PACK SIZES : 1ml | 8ml | 20ml

    Download Protocol

     

    Description:

    HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative  PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.
    HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

  • Probe qPCR Mix Plus (ROX)


    For in vitro use only

    PACK SIZES : 1ml | 8ml | 20ml

    Download Protocol

    Description:

    HOT FIREPol®Probe qPCR Mix Plus (ROX) is optimized for real-time quantitative  PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.
    HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

  • Probe Universal qPCR Mix


    Suitable for ROX-dependent and ROX-independent qPCR cyclers

    For in vitro use only

    PACK SIZES : 1ml | 8ml | 20ml

    Download Protocol

     

    Description:

    5x HOT FIREPol® Probe Universal qPCR Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables efficient amplification of AT-rich, regular and GC-rich (up to 75%) targets. HOT FIREPol® Probe Universal qPCR Mix is optimized for DNA/LNA hydrolysis probes based on the 5´–3´ exonuclease activity.
    HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.