Description
Applications:
– Detection and quantification of DNA and cDNA targets
– Profiling gene expression
– Microbial detection
– Viral load determination
Benefits:
– Highly specific and reproducible real time PCR
– Excellent efficiency in case of low copy number targets
– UNG treatment capability due to dNTP blend of dUTP/dTTP
– Superior performance with long (up to 500 bp) and GC-rich templates
– Blue visualisation dye for easy pipetting
Mix Compatibility:
– Stratagene: MX3000PTM , MX3005PTM
– Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™, Chromo4™, Opticon® 2 & MiniOpticon®
– Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000
– Eppendorf: Mastercycler®: ep realplex2 & ep realplex4
– Roche: LightCycler® 480
Mix Composition:
– HOT FIREPol® DNA Polymerase
– 5x EvaGreen® qPCR buffer
– 12.5 mM MgCl2 1x PCR solution – 2.5 mM
– EvaGreen® dye
– Internal reference based on ROX dye
– GC-rich Enhancer
– Blue visualisation dye
EvaGreen® Dye:
EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!
ROX Dye:
ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.
Shipping and Storage Conditions:
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® qPCR Supermix.
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