Probe qPCR Mix Plus (ROX)


For in vitro use only

PACK SIZES : 1ml | 8ml | 20ml

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Description:

HOT FIREPol®Probe qPCR Mix Plus (ROX) is optimized for real-time quantitative  PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.
HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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Description

Applications:

– Detection and quantification of DNA and cDNA targets

– Profiling gene expression

– Microbial detection

– Viral load determination

 

Mix Composition:

– HOT FIREPol® DNA Polymerase

– 5x Probe qPCR buffer

– 15 mM MgCl2 1x PCR solution – 3 mM

– dNTPs

– ROX dye

 

ROX Dye:

ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.

Shipping and Storage Conditions:

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® Probe qPCR Mix Plus (ROX).

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