PCR Master Mix


with [ 7.5 | 12.5 ] mM MgCl2

For in vitro use only

PACK SIZES : 1ml

Download Protocol

Description:

5x FIREPol® Master Mix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water).

We recommend using 5x FIREPol® Master Mix in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining.

Category: Tags: ,

Description

Applications:

– Suited for a wide range of PCR assays

– Primer extension

– TA cloning

 

Reagent Composition:

– FIREPol® DNA polymerase

– 5x Reaction Buffer B  0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20

– 7.5 mM MgCl2 1x PCR solution – 1.5 mM MgCl2

– 2 mM dNTPs of each  1x PCR solution – 200 µM dATP, 200 µM dCTP,   200 µM dGTP and 200 µM dTTP

Advantages of Master Mix

– decreased set-up time

– reduced risk of contamination

– less pipetting mistakes

– consistent reaction to reaction performance

– contains all reagents required for PCR

Like all our products, the 5x FIREPol® Master Mix is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.

 

Shipping and Storage Conditions:

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8ºC has no detrimental effects on the quality of FIREPol® 5x Master Mix.

Reviews

There are no reviews yet.

Be the first to review “PCR Master Mix”