– Suited for a wide range of PCR assays
– Primer extension
– TA cloning
Storage and Dilution Buffer:
50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
– FIREPol® DNA Polymerase
– 10x Reaction buffer B (Mg2+ free)
0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20
– 10x Reaction buffer BD (Mg2+ and detergent free)
0.8 M Tris-HCl, 0.2 M (NH4)2SO4
– 25 mM MgCl2
– 10x Solution S Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution).
Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.
Shipping and Storage Conditions:
Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol® DNA Polymerase.